Determination of ESR( Erythrofcyte sedimentation rate) by wintrobe hematocrite tube

 

Erythrocyte sedimentation rate

The rate of which RBC is settle out from plasma is the erythrocyte sedimentation rate. The sedimentation rate is measured the suspension stability of erythrocyte. The cells are settle because of their greater density of compared on the plasma.

 

Required material

• Ø  Wintrobe hematocrite tube
• Ø  Special loading pipette
• Ø  Wooden rack
• Ø  Blood sample
• Ø  Cotton

Procedure

• Ø  Take anticogulated well mixed blood into the special loading pipette.
• Ø  Place the tip of the special loading pipette in bottom of the hematocrite tube.
• Ø  Expel the blood slowly by pressing the rubber bulb of pipette and draw up the pipette and fill the blood up to10 or 0 mark.
• Ø  Wipe out the blood above the 10 mark with cotton.
• Ø  The tube is placed in wooden rack and leave for 1 hour.
• Ø  After 1 hour record the ESR value form top of the tube.
• Ø  The result is expressed in mm in first hour.